FastQCFastQC Report
Wed 25 May 2016
SRR1779928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263018
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT158676.032666965758997No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG25520.9702757986145436No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC18350.6976708818407865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12830.48779931411538374No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT10560.40149343390946624TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC6230.23686591792196734No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3610.1372529636754899No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC3290.12508649598126365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG150.002222105470.0070423
GGAGCTA150.00222378269.993731
AAAAACG150.00222378269.9937366
GTCGTCG150.00222378269.9937313
GGTCTCG150.00222378269.9937347
GTATGAC401.063745E-861.24451442
GCAGCGT352.9689363E-759.994621
CTTTATA750.055.9949842
AAGCCGT252.3886938E-455.9949844
AGGGGGG21650.055.61759670
CGTCTTA200.00694334552.5052848
TCTGCGG200.00694856952.49529612
GGCACTC200.00694856952.4952961
GATCATA407.466715E-752.4952961
GTACTAG200.00694856952.4952963
CCGACCC200.00694856952.49529616
GATCTTA200.00694856952.4952961
CACGTAT200.00694856952.49529639
AAAGATC352.0841499E-549.99551833
CGTATGA506.181472E-848.9956141