FastQCFastQC Report
Wed 25 May 2016
SRR1779927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106957
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2858726.72756341333433No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG7280.6806473629589461No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1220.11406453060575746No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAG150.002219787669.9967342
GTTCTAT150.002219787669.996731
TAGGTAC207.897666E-569.9967313
AGTGCCA150.002219787669.9967340
TACTACG150.002219787669.9967323
AGGTACT252.376259E-456.0235814
GGTACTG252.376259E-456.0235815
GATGTAG200.00692335652.522120
ACTGTTT200.00692335652.522118
ACCGTGG200.00692335652.522119
GTTTGCA200.006936179552.49754321
GATACTA200.006936179552.4975431
TCCATAA200.006936179552.49754341
AGTTTGT200.006936179552.49754329
TCGTCTT200.006936179552.49754357
GCCTACA200.006936179552.49754348
CATCCTG200.006936179552.49754346
GACCTCT200.006936179552.49754335
AAGGAGT200.006936179552.49754352
TACTGTT305.84324E-446.6863117
Produced by FastQC (version 0.11.5)