FastQCFastQC Report
Wed 25 May 2016
SRR1779927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106957
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT1650215.428630197182045No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG27002.5243789560290586No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC20381.9054386342174892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13411.2537748814944323No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT7890.7376796282618249TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC5820.544143908299597No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT3070.28703123685219295No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2830.2645923127986013No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT2540.23747861290051142No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2480.23186888188711352No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT1940.18138130276653233No Hit
TTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC1840.17203175107753582No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT1800.16829193040193724No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCAT1780.16642202006413792No Hit
TATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCT1770.16548706489523826No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC1760.16455210972633863No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC1380.12902381330815185No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG1200.11219462026795814No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGA1190.1112596650990585No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACT207.871535E-570.0435219
TGAAATT150.00221390770.0435221
CAGTTGT207.908121E-569.978032
GACGTCA207.908121E-569.9780346
GTGGGCA150.002222141369.9780317
AGGGGGG17800.058.6319370
TGACGTC601.8189894E-1258.3150245
ATCATGT252.3739008E-456.0348130
GTCTTAA252.3793994E-456.00860249
TGCTTAT252.3849081E-455.9824210
TGTAGGG252.3849081E-455.982426
GCCGTAA252.3849081E-455.9824246
TCTTTAT1150.054.765411
CTTTATA1150.054.765412
CCGCCAC200.00691785552.53264222
CTTAAGC200.006930673552.5080751
AGTCCAT200.006943510452.4835235
CTTTTGG200.006943510452.4835216
CTGGTCT200.006943510452.483529
GACCAGG200.006943510452.4835217
Produced by FastQC (version 0.11.5)