FastQCFastQC Report
Wed 25 May 2016
SRR1779926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245858
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2559610.410887585516843No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG5810.23631527141683412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGA150.002225587169.9780351
GTTACCG200.006943013552.50488318
TTACCGC200.006943013552.50488319
TAACCGT200.00694860352.494226
CTTACCG200.00694860352.49429
GATTACG200.00695419652.483532
ACCTAGT352.084005E-549.99447620
TCGCCGT1550.049.6820452
GCCGTAT1550.049.67193654
TGGTCGC1600.048.1294849
TCGGTGG1550.047.42376745
GACAGAC451.679271E-646.6710115
GTCGCCG1650.046.67100551
CTCGGTG1500.046.67100544
CGGTGGT1600.045.94177246
CGCCGTA1750.044.00409353
CTTGGGC404.581701E-543.7540747
GACCTAG404.581701E-543.7540719
CATTGGG404.5872082E-543.745179
TTCAATG404.5872082E-543.7451726
Produced by FastQC (version 0.11.5)