FastQCFastQC Report
Wed 25 May 2016
SRR1779926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245858
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT153846.257270456930423No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG27011.0986016318362632No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC17900.7280625401654613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11790.4795451032709938No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT10260.4173140593350633No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC5110.20784355196902274No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3930.15984836775699796RNA PCR Primer, Index 19 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3410.13869794759576667RNA PCR Primer, Index 19 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCA150.00222238370.0034616
CGTCCAA150.00222238370.0034617
TAACGCC150.00222417769.9892244
AGGGGGG20400.061.42460670
ATTGTCG252.3891464E-455.9913812
CGCGTAA407.44707E-752.51327528
CAAGTGC200.00694421252.50259421
TGGGTCG200.00694421252.50259424
GCTCTAC200.00694421252.5025941
TCCAGTA200.00694421252.50259432
TGGACTA407.4680247E-752.49191744
CGTCTAC200.006949801552.49191748
GTATGAC1050.049.99230242
ACAAGGT506.172377E-849.0024225
CGTATGA1000.048.99245541
TGACGTC1000.048.99245545
CCGTCTT26550.047.97754747
GCCGTCT26700.047.9701446
CGTCTTC27100.047.90775348
TATGCCG27000.047.69635843
Produced by FastQC (version 0.11.5)