FastQCFastQC Report
Wed 25 May 2016
SRR1779925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239465
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3271613.66212181320861No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG7650.31946213434113546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAT150.002222536870.0016713
GGTACCT252.3869208E-456.00133533
CTTCCAT200.00694469552.5012557
TGCTCGA200.00694469552.5012531
AGACCCG200.006950434352.4902886
TTCCGAT503.4687437E-642.00100320
GTAAGGT503.4687437E-642.00100319
GCCGTAT1350.041.4824754
CATCGCA350.001253631540.00095414
TAAGGTG350.001253631540.00095420
TGTATAA459.190098E-538.8816952
TCGCCGT1450.038.62161352
TCCGATA556.6759294E-638.18272821
TCTCGGT1500.037.33422543
CTCGGTG1550.036.12989444
CGGTGGT1550.036.12989446
TCGGTGG1550.036.12989445
TGGTCGC1650.036.06146649
ACGCCAC400.002415339735.00083566
CGTATCA1800.035.00083556
Produced by FastQC (version 0.11.5)