FastQCFastQC Report
Wed 25 May 2016
SRR1779925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239465
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT195128.14816361472449No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG32231.3459169398450714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC20650.8623389639404505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15210.6351658906311987No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT14140.5904829515795628No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC6870.2868895245651765No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3960.16536863424717602No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3620.15117031716534776No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT3340.13947758545090094No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC2670.11149854884847472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTC150.002223563169.9935321
AGGGGGG25950.056.65398870
TGTATAC200.00694215552.5061160
GTTCGAC200.00694215552.5061138
GTATACG200.00694215552.5061161
ACGTATG200.00694215552.5061140
AACTCGT407.4532727E-752.5061137
TATACGG200.00694215552.5061162
CCCGCTG200.00694215552.5061146
AGACTTG200.00694789252.49514432
TGATTCG200.00694789252.49514417
AGGCACT200.00694789252.49514413
CCTTTCG200.00694789252.49514413
GATTCGG200.00694789252.49514418
AAACTCG506.168193E-849.00570336
CGTATGA651.8371793E-1048.4671841
CTTTATA1200.046.6720962
CGTCTTC34900.044.92421348
CCGTCTT34250.044.75498647
GCCGTCT34350.044.7359246
Produced by FastQC (version 0.11.5)