FastQCFastQC Report
Wed 25 May 2016
SRR1779924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277246
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264199.52908247549108No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG6790.24490885350915795No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGC150.00222311970.015
TTCCCCG200.00694650252.527
CCGCCCT200.00694650252.535
CCGTTAG200.00694650252.514
ATAGCGG305.871643E-446.66666436
GCCGTAT1100.044.54545254
TCGCCGT1200.040.83333252
CGCCGTA1200.040.83333253
CTTTTCG350.00125412140.00000448
TCGGTGC350.00125412140.00000455
GTCGCCG1300.037.6923151
CCGTATC1350.036.296355
CGGTGGT1450.036.20689846
CGTATCA1400.035.00000456
TTTTCGC400.002416278535.049
TCGGTGG1600.032.812545
TGGTCGC1500.032.66666849
TCGCTTT652.0983003E-532.30769352
CTCGGTG1550.031.61290444
CGCTCTA450.00430287831.11111333
Produced by FastQC (version 0.11.5)