FastQCFastQC Report
Wed 25 May 2016
SRR1779924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277246
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT157905.695303088232111No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG26480.9551084596351255No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC15840.5713337613527336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13110.4728652532408042No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT7880.2842241186527488TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC5350.19296942065890943No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3140.11325681885401412No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3100.11181405683039611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGC252.8369122E-669.9933249
GCTTTCG308.368128E-658.327778
AGGGGGG19000.057.11012370
TTGCCCG200.006949119752.49499567
TTCGCTC200.006949119752.49499511
TCCCACG200.006949119752.49499514
GCCGTCT24500.047.4240546
CCGTCTT24550.047.3274647
CGTCTTC24700.047.18173648
ATGCCGT26300.046.97270644
TATGCCG26650.046.88108443
TGCCGTC26000.046.84168645
ACGTATG305.869182E-446.6706340
GCCGTAT305.874405E-446.66221646
CGTATGC27150.046.5417141
TCGTATG27850.046.50305640
GTATGCC27000.046.41135442
CTCGTAT27800.046.2089639
CTGCTTG24150.045.9376554
GTCTTCT24350.045.7040649
Produced by FastQC (version 0.11.5)