FastQCFastQC Report
Wed 25 May 2016
SRR1779923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199146
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3429217.219527381920802No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG8580.430839685456901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG200.006934674552.51714717
TACCGGC200.006934674552.51714718
GACGTAT200.0069415752.50395265
AGTGTAC200.0069415752.50395235
CCCTGCG200.0069484752.49077224
CAACGGG200.0069484752.49077229
GGTGAAC200.0069484752.4907722
TGGTGCC200.0069484752.4907728
AATCGAT200.0069484752.49077222
TAATCGA200.0069484752.49077221
TCGCCTA352.0771833E-550.0163318
GATGTCG305.8654515E-446.6701833
ATGTCGG305.8654515E-446.6701834
TCGGTGG2250.040.4474945
TGCGAAG350.001252805240.00301433
ATACCGC350.001252805240.00301467
CTCGGTG2200.039.77572644
GATCTCG2200.039.77572641
TCTCGGT2150.039.0727143
TGGGTCG459.156869E-538.9015914
Produced by FastQC (version 0.11.5)