FastQCFastQC Report
Wed 25 May 2016
SRR1779923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199146
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2156110.826730137687928No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG36411.828306870336336No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC22901.1499101161961576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17300.8687093890914204No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT13730.6894439255621504RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC7510.3771102608136744No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4150.20838982455083205No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3690.18529119339580005No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT2450.12302531810832254RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT2010.1009309752643789No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT207.923809E-569.979914
ATATTCC150.002224727569.97990439
AAACTCG352.969282E-759.9827836
AGGGGGG25850.059.84311370
GGCTTAA308.369003E-658.316591
CGTCTTA252.3866442E-455.9979948
GTACTAG252.389609E-455.983931
ATCGTAT452.694469E-854.4288239
CGTATGA653.6379788E-1253.83070441
AAAGCGT200.006937724552.51130725
TACCACG200.00694462352.49811617
TCGTGAG200.00694462352.49811617
CGGCCTT200.006951526352.4849369
CCTACAT200.006951526352.48493641
TAGCCGG200.006951526352.48493637
AACTCGT407.4676245E-752.48493637
GTAAGGT200.006951526352.4849364
TAGCTTG200.006951526352.48493647
ATCTGCG200.006951526352.48493634
TATGACG750.051.318643