FastQCFastQC Report
Wed 25 May 2016
SRR1779922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357518
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4653413.015848153099984No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG57821.617261228805263No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7770.21733171476680893No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG7650.21397524040747598No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT6270.17537578527514697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA200.006948140452.553
GACGTAT200.006948140452.554
GCCGTAT11700.047.56410254
TCGCCGT11850.047.55274252
CGGTGGT12400.047.137146
TGGTCGC12250.046.85714349
CGTATCA12100.046.57024856
TCGGTGG12500.046.245
ATCTCGG12650.045.65217642
CGCCGTA12350.045.62753353
TCTCGGT12450.045.54216843
CTCGGTG12550.045.45816844
TAGATCT12500.045.3639
GGTGGTC12900.045.31007447
AGATCTC12750.045.29411740
GTATCAT12150.045.22633757
GTGGTCG12950.045.13513648
GATCTCG12800.045.11718841
GTAGATC12650.045.09881638
CCGTATC12350.045.0607355