FastQCFastQC Report
Wed 25 May 2016
SRR1779922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357518
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT323029.035069562931097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100842.820557286626128No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC35030.9798108067286123No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG33770.9445678259556162No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT13630.38123954598090165Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC6470.18096990920736858No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC5320.14880369659709441No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4260.11915483975631995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT207.925706E-569.9918937
AGGGGGG39650.060.90088770
AACTCGT308.374085E-658.32657237
ATCGTAT252.3901698E-455.99350739
ACATTCG200.006951323752.4939210
CACTCGG200.006951323752.4939210
CAACTCG200.006951323752.4939236
AAGGGGG47200.050.9368969
CGTCTTC48800.050.9162348
CCGTCTT48700.050.805247
GCCGTCT48900.050.66897646
CTGCTTG48700.050.5966954
CTTGAAA49450.050.39554657
TCTTCTG49050.050.3713350
TATGCCG49600.050.23611543
TCGTATG49950.050.2344240
CGTATGC49650.050.18552441
CTCGTAT49800.050.1749139
ATCTCGT49850.050.1245837
CTTCTGC49100.050.10620551