FastQCFastQC Report
Wed 25 May 2016
SRR1779921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455052
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368398.095558309819536No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG34310.7539797649499398No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6800.14943347133953921No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG5220.11471216476358745No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAC200.00694943852.4998363
GCCGTAT5950.042.94104454
TCGGTGG6050.041.6527645
TGGTCGC6100.041.31134849
TCGCCGT6200.041.20954552
CTCGGTG6200.041.20954544
CGCCGTA6200.041.20954553
CGGTGGT6150.040.40637646
GATCTCG6550.040.0762141
TCGTTGT350.001254989239.99987435
ATCTCGG6600.039.24230242
GTAGATC6600.039.24230238
TCTCGGT6550.039.0075143
CGTATCA6650.038.94724756
AGATCTC6800.038.6028240
GTCGCCG6550.038.4731651
CCGTATC6850.037.29915255
TAGATCT6950.037.2660739
GTATCAT6950.036.76247457
GTGGTCG7000.035.49988648
Produced by FastQC (version 0.11.5)