FastQCFastQC Report
Wed 25 May 2016
SRR1779921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455052
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT248765.466627989768202No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63771.4013783040180023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC29420.6465195186484183No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG24540.5392790274518078No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT11260.24744424812988405RNA PCR Primer, Index 34 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC5530.12152457301583117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGG150.00222275170.01021631
AGGGGGG32600.057.97118470
CCGTACC200.006948360252.5018961
AAGGGGG37000.050.50978569
GCCGTCT37650.049.8236846
CCGTCTT37300.049.8220647
CGTCTTC37500.049.6496748
CGTATGC38800.049.5195441
TATGCCG38800.049.5195443
TCTCGTA38850.049.00539438
TGCCGTC39000.048.81691445
CTCGTAT39050.048.75440639
TCGTATG39800.048.71500440
ATGCCGT39450.048.7036344
ATCTCGT38950.048.6998737
CTTGAAA39250.048.41681357
GTATGCC39700.048.39693542
GTCTTCT38050.048.2881649
GGATCTC39550.048.13804235
TCTTCTG38150.048.06984750
Produced by FastQC (version 0.11.5)