FastQCFastQC Report
Wed 25 May 2016
SRR1779920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226017
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4052317.92918231814421No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG40091.7737603808563072No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6770.2995349907307857No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG5090.22520429879168383No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4220.18671161903750605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTT207.914863E-570.0000133
TTGTCCC207.914863E-570.0000145
GCGTTTT308.358418E-658.33333212
GCTGCGT252.3869151E-455.99999629
ATCGTGG252.3869151E-455.99999657
CAAAAGT200.006944847852.50000452
GCGAACT407.4573836E-752.50000428
CCGTCTG200.006944847852.50000427
AGACGTG200.006944847852.50000466
AATGCAC200.006944847852.5000043
TCAATCC200.006944847852.50000421
ACCGTCT200.006944847852.50000426
AGACTTC352.0821053E-550.011
TGATCGT305.8693276E-446.66666455
AGCGTTT305.8693276E-446.66666411
CTAAGCG305.8693276E-446.6666648
CTTATAT305.8693276E-446.6666641
TCCAATA305.8693276E-446.66666450
GCGTGCT305.8693276E-446.66666432
TAAGCGT305.8693276E-446.6666649
Produced by FastQC (version 0.11.5)