FastQCFastQC Report
Wed 25 May 2016
SRR1779920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226017
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2738712.117230119858242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72243.196219753381383No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC31511.3941429184530367No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG27881.2335355305131916No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT11130.4924408340965502TruSeq Adapter, Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC4930.21812518527367408No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC4560.20175473526327664No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3980.17609294876049147No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2920.12919382170367716No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2790.12344204197029428No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG207.920442E-569.9900436
GATACGC150.0022238469.990048
GGGACAA150.0022238469.9900458
ACGCTGA150.0022238469.9900437
AACTCGT207.920442E-569.9900437
AGGGGGG33300.063.05409270
ACTCTCG308.365467E-658.3250438
ATCGTAT850.057.63886339
CGAATGC252.3885934E-455.9920341
CATCGAA252.3885934E-455.9920341
CGCAAAC252.3885934E-455.9920350
AAGGGGG38850.053.8662169
CGTCTTC40700.052.6215148
CCGTCTT40600.052.5787347
GGGAGCG200.006936602352.515772
GCCGTCT41000.052.49253546
TGATACG200.006948756552.492537
CTAACTG200.006948756552.4925337
ACTGTAC200.006948756552.492538
AATCTCG40500.052.44932636
Produced by FastQC (version 0.11.5)