FastQCFastQC Report
Wed 25 May 2016
SRR1779919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183626
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3583819.516844019910035No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG35931.9566945857340465No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC4970.2706588391622101No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4470.24342957968915077No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG4360.2374391426050777No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA150.002221988769.999452
TACCGAC200.006935520652.51389369
CAGTTGT200.006942997752.49958815
TCCACGG200.006942997752.49958830
TGCACTA200.006942997752.49958851
TATAGTC305.8666593E-446.66635
GCCGTAT6250.044.79965254
CGTATCA6400.044.29652856
CGGTGGT6500.044.15350346
CCGTATC6350.044.09414755
CGCCGTA6350.044.09414753
GTATCAT6450.043.95314457
TGGTCGC6400.043.74965749
TCGCCGT6350.043.5429752
ATCTCGG6400.043.20278542
CTCGGTG6400.043.20278544
TCGGTGG6650.043.1575645
GATCTCG6500.042.5381341
AGATCTC6550.042.21341340
CATTAAA6750.041.99967261