FastQCFastQC Report
Wed 25 May 2016
SRR1779919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183626
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2490413.56234955834141No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62583.4080141156481107No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC27501.4976092710182654No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG23811.2966573361070872No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT6930.3773975362966029RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC4340.23634997222615534No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3900.21238822388986311No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2700.1470380011545206No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2340.12743293433391786No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2150.11708581573415529No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC150.002222159669.9980934
CTAAGTT150.002222159669.998099
AACGAAT150.002222159669.998091
TGGATAC150.002222159669.9980937
AGGGGGG30150.063.61352570
AAGGGGG35400.054.0804569
CTGCTTG35700.053.8220654
CGTATGC36850.053.75701541
GCCGTCT36350.053.72618546
CTTGAAA36500.053.6971757
CCGTCTT36250.053.68129747
CGTCTTC36200.053.65875648
GCTTGAA36550.053.62370756
GTCTTCT36050.053.59077549
TGCTTGA36200.053.56207355
TCTTCTG35950.053.5451350
TTGAAAA36900.053.4944858
ATGCCGT36900.053.4944844
TATGCCG37050.053.4668343
GTATGCC36950.053.42209242