FastQCFastQC Report
Wed 25 May 2016
SRR1779918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158956
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4075325.6379123782682No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG35692.2452754221293945No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC5370.33782933642014146No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4240.26674048164271874No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG3520.2214449281562193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA207.9088815E-569.997854
CAGCACA150.002221646769.99779530
TGTGCGC150.002221646769.9977953
TCTAAAG150.002221646769.9977958
TCGCGAC150.002221646769.9977958
GTAAATC150.002221646769.9977953
CTTTATA308.348612E-658.3314972
ACACGAC252.3851226E-455.9982386
ACTTACC252.3851226E-455.99823845
CCATGTT252.3851226E-455.99823850
TTACGAC252.3851226E-455.99823855
ATTTCGG252.3851226E-455.99823848
ATCGAGC200.006933304552.5148718
TGACAGC200.006941939752.4983536
GCAGCAC200.006941939752.4983529
ATGCACG200.006941939752.4983533
TGCACGC200.006941939752.4983534
TGCCACT352.0796753E-549.99843239
GTACACT305.864952E-446.66519541
TACGACT305.864952E-446.66519556
Produced by FastQC (version 0.11.5)