FastQCFastQC Report
Wed 25 May 2016
SRR1779918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158956
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT1711310.765872316867561No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64704.0703087646895995No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT27361.7212310324869775No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG14260.8971036009965022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC11690.7354236392460807No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT6230.3919323586401268No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT5030.31643976949596114No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT4540.2856136289287602No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG3900.24535091471853845No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT3540.22270313797528876No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT3080.1937643121366919No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT2820.17740758448878935No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT2410.15161428319786607No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT2330.14658144392158837No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT2230.14029039482624123No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2180.13714487027856767No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT2110.13274113591182465TruSeq Adapter, Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCC2030.12770829663554695No Hit
CTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC1980.12456277208787336No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG1970.12393366717833867No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCAT1660.10443141498276254No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA1620.10191499534462367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG150.002217884270.027729
CCTAAGG150.002220655370.005662
GGTAAGT150.00222342969.9836412
GCCGGCA150.00222342969.9836446
CATCTAA150.00222342969.9836436
CGAGGCA301.02027116E-769.9836431
TCGAGGA150.00222342969.9836438
TCGGATG501.4551915E-1162.9852840
AAGCCGT850.061.75027544
TAAGCCG850.061.75027543
CACGTAT401.0611984E-861.23568739
AGGGGGG24700.060.79438870
TTCGAGG352.951474E-760.02374629
GCCGACA352.9643525E-759.98598546
TCTCGGA650.059.2169338
AGCCGTC601.8189894E-1258.31970245
TGCCGGA601.8189894E-1258.31970245
CTTAAGC2650.058.11791251
GTCTTAA2650.058.11791249
ACGTAAT553.6379788E-1157.25934647
Produced by FastQC (version 0.11.5)