FastQCFastQC Report
Wed 25 May 2016
SRR1779917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172951
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3422619.789420124775226No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG35162.0329457476395048No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC4940.28563003394024894No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4050.2341703719550624No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG4010.2318575781579754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT150.00222195669.9979852
CAGTTGT150.00222195669.997981
TAACGAG150.00222195669.9979866
CTAACGC150.00222195669.9979863
GTGTGCG252.385683E-455.998382
TGACCGA200.006934962752.51366418
TGCCCCG200.006942952.498489
GTACAAG200.006942952.498481
CGTTGAT200.006942952.4984837
GATCCTA200.006942952.498481
CGTTCCA200.006942952.4984813
CGTACAC200.006942952.498483
GCCGTAT4950.050.20056554
TGGTCGC4950.049.49351549
CTCGGTG5100.049.41033644
CGGTGGT5100.049.41033646
ATCTCGG5250.049.33190542
GATCTCG5250.049.33190541
TCGCCGT4850.049.07074752
CGCCGTA5100.048.7240853
Produced by FastQC (version 0.11.5)