FastQCFastQC Report
Wed 25 May 2016
SRR1779917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172951
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2245412.982867979948079No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61183.537418112644622No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC26241.5171927308890958No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG23701.370330324774069No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT8870.5128620245040503No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT8800.508814635359148Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC5110.29545940757786887No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3730.21566802157836612No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2760.1595827719990055No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2430.14050222317303745No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2110.12199987279634117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA150.002220681470.00809548
GCAAATG150.002223230469.9878541
AAGGTCA150.002223230469.9878545
AACTCGT150.002223230469.98785437
AGGGGGG28150.062.9202470
GCACCAT308.358744E-658.3232132
AAGGGGG33050.052.8502969
CAAAAGT200.00693099352.5212623
TAACGTT200.00693892852.50607348
GATAACG200.006946869752.49089446
TCCGATA200.006946869752.49089415
CCGATAA200.006946869752.49089416
CGATAAC200.006946869752.49089445
CCGTCTT34900.052.24022347
CGTCTTC35000.052.1060348
TATGCCG35600.051.99940543
GCCGTCT35200.051.99381646
TCGTATG35900.051.85730440
GTATGCC35650.051.82831242
CGTATGC35750.051.78122741
Produced by FastQC (version 0.11.5)