FastQCFastQC Report
Wed 25 May 2016
SRR1779916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766896
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335754.378038221610232No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG33500.43682585383154954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT150.002224555770.000525
GGTACGT252.3912164E-455.9967636
GCCGTAT7700.038.18210254
CGTATCA8000.037.18777556
TCGGTGG8100.037.15834845
TCGCCGT7850.037.0042352
TGGTCGC8050.036.95438849
GTCGCCG8150.036.50095751
CGCCGTA8150.036.07388753
CTCGGTG8150.036.07153344
CGGTGGT8250.035.63430846
CCGTATC8400.035.41692755
TCTCGGT8350.035.20984343
AACGTTC400.00241961834.99798212
TGGCGAT400.00241961834.99798235
TTCCGAC400.00241961834.99798252
GTGGTCG8750.034.3980148
GGTCGCC8750.033.99803550
GTAGATC9650.031.91733738
GTATCAT9250.031.78402357
Produced by FastQC (version 0.11.5)