FastQCFastQC Report
Wed 25 May 2016
SRR1779916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766896
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT217312.8336306357054935No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65510.8542227368508898No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC28810.3756702342951326No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG21360.27852538023408646No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT9300.12126807285472868TruSeq Adapter, Index 9 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT252.3877836E-456.01304227
AGGGGGG27700.055.7277370
AAGGGGG31900.047.84189669
CCGTCTT32750.046.80480247
GCCGTCT33100.046.62707546
CGTCTTC33200.046.38726848
CGTATGC33850.046.21430641
CTCGTAT34000.046.1133539
TCGTATG34250.046.08329840
TATGCCG33600.046.03737643
TGCCGTC33550.046.0016745
ATCTCGT34250.045.77675637
TCTCGTA34450.045.71417638
ATGCCGT33850.045.69736544
GTATGCC34150.045.39840742
GATCTCG36550.043.6621436
AGATCTC36250.043.4442335
GTCTTCT35800.042.62728549
TAAGATC36200.042.5402533
CTGCTTG36000.042.1960254