FastQCFastQC Report
Wed 25 May 2016
SRR1779915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331937
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3450210.3941410568873No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG39711.196311348237768No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6740.20305057887490696No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG5030.15153477919002703No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4390.1322540120565047No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGA150.002223544569.999722
GTCGTCT150.002223544569.999761
CGCCGTG150.002223544569.999722
TCGTCTC200.006947821552.49977562
AGTCGTC200.006947821552.49977560
ACCGACC305.873453E-446.6664668
CGAATTC451.6821941E-646.6664669
GCGATCT305.873453E-446.66646613
GTTCGAA404.5878278E-543.7498136
TGTTCGA404.5878278E-543.7498135
TCGGTGG7800.041.7305945
TCGCCGT7600.040.98666452
CTCGGTG7950.040.9432244
TCTCGGT8050.040.8693943
GCCGTAT7650.040.7187854
CGGTGGT8000.040.6873346
CGTATCA7800.040.3844456
CATTCGA350.001254505239.999839
CCGACTA350.001254505239.9998336
TGGTCGC8050.039.9998349