FastQCFastQC Report
Wed 25 May 2016
SRR1779915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331937
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT230296.937762286216962No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68792.0723812048671886No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC25960.7820761168534992No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG24600.7411044866947644No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT8080.24341968506071937TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC5460.16448904460786234No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC3820.11508207882821138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG29200.059.44911270
GCCGTCT33350.052.7859946
CCGTCTT33450.052.52355647
TCGGGTC200.00694492452.505316
GGCCGAC200.00694906452.497416
CGAATGC200.00694906452.497441
CGTCTTC33100.052.23306348
TCTTCTG32550.051.395350
TCTCGTA36500.051.39471438
CGTATGC35300.051.35723541
TATGCCG35650.051.34388443
GTCTTCT32650.051.2378949
CTCGTAT36000.051.23357439
TCGTATG36000.051.23357440
TGCCGTC35600.051.21937645
CTTCTGC32750.051.18830551
ATCTCGT36700.051.11463537
CTGCTTG32900.051.061354
GTATGCC36050.050.7741942
ATGCCGT36200.050.75715344