FastQCFastQC Report
Wed 25 May 2016
SRR1779914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369678
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG332598.99674852168644No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG38371.0379303069157484No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC5730.15499975654488501No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG5620.15202419402831654No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4630.12524413137920026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG207.9215126E-570.0000155
GCTACGG150.002223707570.01
TCGTTGC200.006948324452.50000412
CGTTGCA200.006948324452.50000413
AACGATA200.006948324452.50000423
CAGCGTT350.001254664340.053
GTTCTAT350.001254664340.01
ACGATGT350.001254664340.030
CGTTGAG459.18979E-538.88888549
GCCGTAT6500.037.6923154
TCGCCGT6800.036.54411752
CGCCGTA6800.036.54411753
CGGTGGT6750.036.29629546
CTCGGTG6700.036.04477744
AGATCTC7000.036.040
GATCTCG7050.035.7446841
TGGTCGC7050.035.7446849
TCGGTGG6850.035.25547845
TCTCGGT6950.035.251843
ACGTTTA400.002417317435.00000427