FastQCFastQC Report
Wed 25 May 2016
SRR1779914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369678
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT225176.090976471415664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69591.882449050254546No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC31420.8499288570052856No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG21460.5805051964141766No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT9110.24643067750853448No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC4540.12280958022928062No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTC150.002224474269.9939156
AGGGGGG29700.058.68176770
CAGTTCG252.3899347E-455.99513242
TCGAGAT200.006950713752.49543846
TTCGAGA200.006950713752.49543845
CGCTTCT200.006950713752.4954384
CGTATGA608.367351E-1152.49543441
AAGGGGG33650.051.48140769
TCGTATG36350.050.5458140
CCGTCTT34700.050.52874847
TATGCCG35550.050.50193843
CGTATGC35850.050.46980741
GCCGTCT35050.050.42357346
CTCGTAT36400.050.2840939
CGTCTTC35350.050.19365748
GTATGCC35900.050.0095842
CGTCTAC352.0857753E-549.9956548
TGCCGTC35800.049.85599545
ATGCCGT35950.049.84267444
TCTCGTA36950.049.63032538
Produced by FastQC (version 0.11.5)