FastQCFastQC Report
Wed 25 May 2016
SRR1779913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378513
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4525511.955996227342258No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG49471.306956432143678No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6410.16934689165233427No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT5050.1334168179164256No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG4940.13051070901131534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTC150.002223781569.9997317
CGTGCGA308.369469E-658.33311510
GCGAGAA352.084845E-549.99981313
AAGAGAC1050.046.6664925
CTTTATA703.8380676E-1044.9998322
CCGTGCG404.5890418E-543.7498369
CGAGAAT404.5890418E-543.74983614
TCGCCGT8250.043.27256452
GCCGTAT8300.043.01188754
CGGTGGT8800.042.55665646
ATCTCGG8600.042.3254242
TGGTCGC8700.042.24122249
GATCTCG8650.042.08076541
CGTATCA8750.041.99984456
TCGGTGG8800.041.76120445
GTCGCCG8700.041.43662651
CTCGGTG8850.041.1297944
AGATCTC9000.040.8331840
GTAGATC9150.040.5462938
TCTCGGT9150.040.1637843