FastQCFastQC Report
Wed 25 May 2016
SRR1779913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378513
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT314358.304866675649185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84992.2453654167756456No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG33990.8979876516790706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC27110.7162237492503559No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT11780.3112178445654442No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5540.14636221213009856No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4540.11994304026545985No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC4310.11386663073659294No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGC150.002224813769.9915538
TACGCGG150.002224813769.991555
AGGGGGG38550.061.82132370
CCTTATA308.375275E-658.326291
ATCGTAT308.375275E-658.3262939
GGAATAT252.390408E-455.9932331
ATGTCGC252.390408E-455.99323337
AAGGGGG44450.053.22191669
TATGCCG46350.052.5502943
AACGGGT200.0069336352.52835524
AAGGATC200.0069336352.52835528
ACTGTGC200.006944511652.5075333
CATAGCG200.00695177352.4936640
ATGACGA200.00695177352.4936644
CGTCTTC46250.052.43691348
CGTATGC47000.052.41919741
TCGTATG47150.052.40088340
CCGTCTT46200.052.34216347
GCCGTCT46350.052.3237846
TCTTCTG46400.052.26739550