FastQCFastQC Report
Wed 25 May 2016
SRR1779912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343637
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3775410.986593411070404No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG40191.169548098720452No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC6970.20283031221899855No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT4160.1210579768767624No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG3640.1059257297671671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGA207.917866E-570.005119
ACTCGGT150.002222931570.005143
TGGAATC150.00222421569.994968
CGTTGGA200.006945911352.5038214
CAACTCG200.006945911352.5038228
CTAAGTC200.00694991152.4961839
CTATCGG451.6814738E-646.67006738
TCGGGCC451.6814738E-646.67006741
CTGGTCA451.6814738E-646.67006717
GATCCAC305.875725E-446.663271
GATCATA305.875725E-446.663271
ACATGTT551.3082717E-744.54869529
TGGTCGC7000.044.00320449
CTCGGTG7400.043.98968544
TCGGTGG7400.043.98968545
CGGTGGT7250.043.9342346
TCGCCGT6900.043.1190852
ATCTCGG7350.042.86026442
GATCTCG7350.042.86026441
GCCGTAT6950.042.80264354