FastQCFastQC Report
Wed 25 May 2016
SRR1779912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343637
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT263157.657790051711544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69932.0349962314884604No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC26890.7825117784173415No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG26010.7569033602318725No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT8970.2610312626405189TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC4960.14433835704537054No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3880.11290984381774954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA150.002223206770.0029141
AGAGTTG150.002223206770.00291422
GCCGACT150.002224490369.9927246
CACTTGA150.002224490369.9927236
GCCGTAT550.063.6297546
AGGGGGG33000.061.29665470
GTATTCT553.6379788E-1157.26677349
CAGGTAT252.388179E-456.0023271
TGCCGTA700.054.9942845
AAGGGGG37900.053.09474669
CGAGTTG200.006950767252.4945420
TATGCCG39950.052.2098443
CGTCTTC38600.052.1318948
GCCGTCT38600.052.1318946
CCGTCTT38550.052.10872347
CGTATGC40100.051.9272741
ATGCCGT40200.051.88515544
GTATGCC40450.051.73751442
TGCCGTC39600.051.6991745
TCTTCTG38900.051.63987450