FastQCFastQC Report
Wed 25 May 2016
SRR1779911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71502
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4348960.8220749069956No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG64118.966182764118487No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC7050.985986405974658No Hit
TCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGT6870.9608122849710499No Hit
CTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG5180.7244552599927275No Hit
TCTTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGG1580.22097283992056166No Hit
CTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG1390.19440015663897514No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1330.1860087829711057No Hit
CTTATACACATCTGACGCTGCCGCCGATCTACTCTGTGTAGATCTCGGTG1170.1636317865234539No Hit
TATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGT950.13286341640793264No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG810.11328354451623732No Hit
CTTTACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGG770.10768929540432436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT252.8138202E-670.000011
CACATCA207.877955E-570.07
TCGACGA150.002216357570.049
CTTTACA150.002216357570.01
AGGATCA150.002216357570.06
TAAGCAG150.002216357570.011
TTACACA150.002216357570.03
GGATCAA150.002216357570.07
CATCAGA150.002216357570.09
AGCAGCA150.002216357570.013
ATCAGAC150.002216357570.010
TCAGACG150.002216357570.011
AGGTCGA150.002216357570.046
ACAAGCT150.002216357570.035
GGGCGCT150.002216357570.013
CAAGCTG150.002216357570.036
AAGCAGC150.002216357570.012
GCGCTGC401.0482836E-861.2515
CTGCCGG308.300329E-658.33333218
AAGGGGG252.3758507E-456.00000470
Produced by FastQC (version 0.11.5)