FastQCFastQC Report
Wed 25 May 2016
SRR1779911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71502
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3120843.64633157114486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1046614.637352801320242No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG34034.759307431959945No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC23543.292215602360773No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT10301.4405191463175855RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC6850.9580151604150933No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC5070.7090710749349668No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4540.6349472742021203No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3300.4615255517328187No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2880.40278593605773266No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC2340.3272635730469078RNA PCR Primer, Index 19 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2270.31747363710106014No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC2160.3020894520432995No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT1920.2685239573718218RNA PCR Primer, Index 19 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC1900.2657268328158653No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT1410.19719728119493163No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC1270.17761740930323627No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1130.15803753741154095No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1110.15524041285558446No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT1110.15524041285558446No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG880.12307348046208498No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT820.11468210679421555No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT810.11328354451623732No Hit
TCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT720.10069648401443317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA252.8138202E-670.0000124
TCTTAAT252.8138202E-670.000011
GGCACCC150.002216357570.07
CTTTACA353.6470738E-970.01
AAATACA150.002216357570.02
CCGTCTA150.002216357570.047
TCTACTG150.002216357570.050
GTCTACT150.002216357570.049
TCTTTAT1100.070.01
CTTTTTG150.002216357570.01
AGGGGGG35850.063.75174370
TTTATAC1350.062.2222253
CTTTATA1250.061.6000022
TTACACA401.0482836E-861.253
TTTACAC401.0482836E-861.252
TCTATAC352.9363764E-760.01
GACATAT308.300329E-658.33333226
ACATATG308.300329E-658.33333227
ATATGAA308.300329E-658.33333229
AGACTAA308.300329E-658.33333225
Produced by FastQC (version 0.11.5)