##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71502 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.739335962630417 32.0 27.0 32.0 13.0 32.0 2 30.24691617017706 32.0 32.0 32.0 27.0 32.0 3 30.416939386310872 32.0 32.0 32.0 27.0 32.0 4 31.190512153506194 32.0 32.0 32.0 32.0 32.0 5 31.12842997398674 32.0 32.0 32.0 32.0 32.0 6 34.983496965119855 37.0 37.0 37.0 32.0 37.0 7 35.12919918323963 37.0 37.0 37.0 32.0 37.0 8 35.04425051047523 37.0 37.0 37.0 32.0 37.0 9 35.35969623227322 37.0 37.0 37.0 32.0 37.0 10-11 35.43121870716903 37.0 37.0 37.0 34.5 37.0 12-13 35.567012111549325 37.0 37.0 37.0 32.0 37.0 14-15 35.51850297893765 37.0 37.0 37.0 32.0 37.0 16-17 34.07393499482532 37.0 34.5 37.0 24.5 37.0 18-19 34.75658023551789 37.0 37.0 37.0 27.0 37.0 20-21 35.50406981622892 37.0 37.0 37.0 32.0 37.0 22-23 35.011160526978266 37.0 37.0 37.0 29.5 37.0 24-25 35.376954490783476 37.0 37.0 37.0 32.0 37.0 26-27 35.63213616402338 37.0 37.0 37.0 32.0 37.0 28-29 35.70553970518307 37.0 37.0 37.0 32.0 37.0 30-31 34.92372940617045 37.0 34.5 37.0 29.5 37.0 32-33 35.33717937959777 37.0 37.0 37.0 32.0 37.0 34-35 35.18625353136975 37.0 37.0 37.0 32.0 37.0 36-37 34.15717042879919 37.0 34.5 37.0 27.0 37.0 38-39 34.588773740594675 37.0 37.0 37.0 27.0 37.0 40-41 35.047243433750104 37.0 37.0 37.0 32.0 37.0 42-43 35.111989874409105 37.0 37.0 37.0 32.0 37.0 44-45 35.00143352633493 37.0 37.0 37.0 32.0 37.0 46-47 34.85522083298369 37.0 37.0 37.0 29.5 37.0 48-49 33.98636401778971 37.0 37.0 37.0 24.5 37.0 50-51 34.42823277670554 37.0 37.0 37.0 27.0 37.0 52-53 34.03844647702162 37.0 37.0 37.0 24.5 37.0 54-55 34.14323375569914 37.0 37.0 37.0 27.0 37.0 56-57 34.038887024139186 37.0 37.0 37.0 24.5 37.0 58-59 34.083913736678696 37.0 37.0 37.0 27.0 37.0 60-61 34.047991664568826 37.0 37.0 37.0 24.5 37.0 62-63 34.482818662415035 37.0 37.0 37.0 27.0 37.0 64-65 34.56815193980588 37.0 37.0 37.0 27.0 37.0 66-67 34.42983413051383 37.0 37.0 37.0 27.0 37.0 68-69 33.83834018628849 37.0 34.5 37.0 24.5 37.0 70-71 31.46456742468742 37.0 29.5 37.0 13.0 37.0 72-73 33.15126849598613 37.0 34.5 37.0 17.5 37.0 74-75 34.331102626499955 37.0 37.0 37.0 27.0 37.0 76 34.54067019104361 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.7432815543158249 11101 2 0.07271338639550251 11101 3 -1.131494321140103 11101 4 -0.05585623049831412 11101 5 0.33063902606689055 11101 6 -0.9463046510768223 11101 7 0.3500944657423304 11101 8 -0.8059203995473041 11101 9 0.10120936831201988 11101 10-11 -0.05889144379862188 11101 12-13 -0.42221522780672416 11101 14-15 0.5534442831960007 11101 16-17 -1.351437134664998 11101 18-19 -0.40188095897453735 11101 20-21 0.846548520600571 11101 22-23 0.1812979362558238 11101 24-25 0.18418995086239676 11101 26-27 0.44450385996625386 11101 28-29 0.011479816132776932 11101 30-31 -0.2863599895832394 11101 32-33 0.2559699661759396 11101 34-35 0.28381621435664073 11101 36-37 -0.09048277795996285 11101 38-39 -0.9857808619156074 11101 40-41 0.31526242744334354 11101 42-43 0.22681211849173621 11101 44-45 -0.26537246881797216 11101 46-47 -0.22109579995461814 11101 48-49 -1.1610014834179196 11101 50-51 0.079545400190284 11101 52-53 -0.42491948927631995 11101 54-55 0.132876530926211 11101 56-57 0.051534145271219245 11101 58-59 -1.3787318292963846 11101 60-61 -0.5293943329584891 11101 62-63 -0.9030458858476038 11101 64-65 0.34252027551016084 11101 66-67 -0.4224572739930892 11101 68-69 -0.9034596633391843 11101 70-71 -1.6022963315049807 11101 72-73 -0.7430918885371796 11101 74-75 -0.25051008080144754 11101 76 0.7918325622950277 11102 1 0.2321901612368542 11102 2 0.46224311132895224 11102 3 0.5858969832077214 11102 4 -0.003948510888729828 11102 5 -0.02384189673701087 11102 6 0.1135888715496236 11102 7 -0.4764361465025644 11102 8 -0.45986893548342067 11102 9 0.1997009388532831 11102 10-11 -1.3046767144286093 11102 12-13 -0.14382126152455754 11102 14-15 -0.4478866839734792 11102 16-17 -0.6511265756587861 11102 18-19 0.3046407801558999 11102 20-21 0.24295490924298946 11102 22-23 0.15356945355839713 11102 24-25 0.14004620640809406 11102 26-27 -0.20101521722983762 11102 28-29 0.29342302820020905 11102 30-31 0.10161694031915403 11102 32-33 0.5410187683054914 11102 34-35 0.5804000652883161 11102 36-37 -2.2413257238339632 11102 38-39 -1.4360914209218194 11102 40-41 -0.5625104208264062 11102 42-43 -0.25100509535032245 11102 44-45 0.3477597405875201 11102 46-47 0.13005770492559066 11102 48-49 0.316815730424139 11102 50-51 0.36925258741299416 11102 52-53 0.17379390912652326 11102 54-55 -0.4491997778581691 11102 56-57 0.3332555294770785 11102 58-59 0.23240392935490206 11102 60-61 0.17668375044878815 11102 62-63 0.356049056477147 11102 64-65 -0.1699020847382826 11102 66-67 0.6230174376306863 11102 68-69 -0.32404528889375683 11102 70-71 -0.30460334126540545 11102 72-73 0.3429773217556331 11102 74-75 -6.642507828757971E-5 11102 76 1.407183050316327 11103 1 -0.33531376340992836 11103 2 -8.652089222245252E-4 11103 3 -0.37564147833073847 11103 4 -0.7849532204648675 11103 5 0.31642497924415025 11103 6 -0.19881301228083714 11103 7 0.1385560042038705 11103 8 0.15394582118847921 11103 9 0.5560588666397805 11103 10-11 0.17020554616794215 11103 12-13 0.2659118624942778 11103 14-15 0.5413205373766061 11103 16-17 -0.7527749273071365 11103 18-19 -0.8492053736902534 11103 20-21 -0.2909932854194892 11103 22-23 -0.25557497344317426 11103 24-25 0.06253443246774992 11103 26-27 -0.04128209321100229 11103 28-29 0.11014202349063851 11103 30-31 -0.2474803909210337 11103 32-33 0.0431773240354687 11103 34-35 -0.6860834511952021 11103 36-37 -0.7209175605686582 11103 38-39 -0.9305969154273086 11103 40-41 0.016767444165751044 11103 42-43 0.8840027539432498 11103 44-45 0.22668438736931762 11103 46-47 0.43107811308885857 11103 48-49 -0.010499811177119511 11103 50-51 -0.4898525931207587 11103 52-53 -0.19080577690173328 11103 54-55 -0.7429595894751273 11103 56-57 1.120932138582262 11103 58-59 -0.03466828414588008 11103 60-61 -1.0929394834601638 11103 62-63 -1.8185977253124932 11103 64-65 -0.2866603264965306 11103 66-67 -0.12521647131750768 11103 68-69 -1.1191787268843356 11103 70-71 -1.3970287729765474 11103 72-73 -0.48473068452379664 11103 74-75 0.8368645011383578 11103 76 -1.1253915179725311 11104 1 -0.625821128385379 11104 2 -0.10397486522336763 11104 3 -0.49920939977100076 11104 4 -0.12292376056306864 11104 5 0.04433005289390479 11104 6 -0.053150164490325835 11104 7 0.296902976380629 11104 8 0.37816840711319344 11104 9 0.56096885027317 11104 10-11 0.33796332030869536 11104 12-13 -0.009865551581007992 11104 14-15 -0.0853161238345308 11104 16-17 0.42030199576978333 11104 18-19 0.16634290781546923 11104 20-21 -0.3844875571052526 11104 22-23 0.7000305912049498 11104 24-25 0.7362251034988461 11104 26-27 -0.4287616349458645 11104 28-29 -0.23805764917712224 11104 30-31 0.48676674492184446 11104 32-33 0.2451004009585489 11104 34-35 -1.2691440076600102 11104 36-37 0.48121009900581413 11104 38-39 -0.35858382213762496 11104 40-41 -0.9365877113170669 11104 42-43 1.039485569000533 11104 44-45 0.9441557457981204 11104 46-47 0.042780240748435006 11104 48-49 0.8561123001158393 11104 50-51 0.9745500255371837 11104 52-53 -0.8303311451505095 11104 54-55 0.6078996576607167 11104 56-57 1.209721009286028 11104 58-59 -0.1379825230984224 11104 60-61 0.03512925631070374 11104 62-63 1.5132353352439765 11104 64-65 0.06869973896991866 11104 66-67 1.0259701080605623 11104 68-69 0.3546346937375944 11104 70-71 0.6997388211314686 11104 72-73 0.0066768441177771365 11104 74-75 0.9499987073576648 11104 76 -0.0014144312294845918 11105 1 0.9260677122572609 11105 2 -0.21122627642614944 11105 3 -0.29165403720044125 11105 4 -0.49374442925607553 11105 5 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0.7790775070984495 11105 66-67 0.16383376416129636 11105 68-69 1.3698322621266499 11105 70-71 -0.4168482392245849 11105 72-73 -0.22845124967293629 11105 74-75 -0.15312764956807712 11105 76 1.2031014176632624 11106 1 0.7065665784704116 11106 2 -0.6094122174692309 11106 3 -0.14743635012561285 11106 4 -0.6302523657640116 11106 5 0.11856173138090043 11106 6 0.6986228851550607 11106 7 -1.4165722009243353 11106 8 -0.7759687087260474 11106 9 -0.6900949795140647 11106 10-11 0.44883802480041624 11106 12-13 -1.0528915563091417 11106 14-15 -1.9571837361276678 11106 16-17 0.686968662436449 11106 18-19 -0.028692553177428692 11106 20-21 -0.25659157601777594 11106 22-23 0.261732718864522 11106 24-25 -1.036824541891228 11106 26-27 -0.16564106756997177 11106 28-29 -0.07233660898799599 11106 30-31 -1.5382923567330025 11106 32-33 -0.4215662657081225 11106 34-35 0.32801911290736285 11106 36-37 -1.676473116124214 11106 38-39 -0.8964088812392745 11106 40-41 -0.8328052054068991 11106 42-43 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11107 22-23 -0.5320172811354809 11107 24-25 -0.04724120855789238 11107 26-27 -0.2760577342366375 11107 28-29 -0.0396977200991131 11107 30-31 -0.1369034678441139 11107 32-33 0.2138504009585489 11107 34-35 0.65926911290736 11107 36-37 0.3207491060980061 11107 38-39 -0.038769992350388804 11107 40-41 0.47830590570421094 11107 42-43 -0.3692023742618886 11107 44-45 0.2907869514718726 11107 46-47 0.7331614464221943 11107 48-49 0.4558463426690338 11107 50-51 0.14476279149462812 11107 52-53 -0.281350648696602 11107 54-55 0.1672968207812815 11107 56-57 0.03840008730020372 11107 58-59 0.1760688953412881 11107 60-61 0.6594643626936829 11107 62-63 -0.8482451612099311 11107 64-65 -0.2041282752144724 11107 66-67 0.7577963491953099 11107 68-69 0.2582251192695111 11107 70-71 1.4683558424080658 11107 72-73 0.14187187957875835 11107 74-75 -1.0765970373231823 11107 76 0.07172386664285568 11108 1 -0.1670445326406984 11108 2 -1.6691344396914545 11108 3 -2.5391030167922786 11108 4 0.6878031897915413 11108 5 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0.11015142165908287 21611 68-69 0.19436642361733902 21611 70-71 1.7563993206689332 21611 72-73 1.7134298505932577 21611 74-75 1.2820986148507245 21611 76 0.7048760405559023 21612 1 2.0500009219047577 21612 2 -0.4498162578732732 21612 3 0.16771516502590345 21612 4 0.0828789473672984 21612 5 0.3286627414819101 21612 6 0.6137744003065748 21612 7 0.8682762839241462 21612 8 0.5007989680416287 21612 9 0.8008141113950273 21612 10-11 0.08419156015395401 21612 12-13 -0.5417804451980288 21612 14-15 -0.3501130290569563 21612 16-17 0.5112110866788768 21612 18-19 1.2319135074286294 21612 20-21 0.333307413881208 21612 22-23 -0.02917637204457435 21612 24-25 0.23893303386635267 21612 26-27 0.7737528718239659 21612 28-29 0.21049167384028067 21612 30-31 0.2687783503377048 21612 32-33 0.32691858277672736 21612 34-35 0.15832214321039118 21612 36-37 0.19726425761316335 21612 38-39 0.8924800076496169 21612 40-41 -0.0671486397503358 21612 42-43 0.4951915651320533 21612 44-45 -0.3630009273160013 21612 46-47 0.09016902217976508 21612 48-49 -0.1384718391491475 21612 50-51 0.49930824604008706 21612 52-53 0.6116417755458201 21612 54-55 -0.2864910580065967 21612 56-57 -0.659137791487673 21612 58-59 -0.8362417107193068 21612 60-61 0.7759416354209563 21612 62-63 0.4348987781840137 21612 64-65 0.7275005126643066 21612 66-67 0.5445387734377363 21612 68-69 1.4906114829058765 21612 70-71 2.1660831151353364 21612 72-73 0.31422036442724277 21612 74-75 0.29158478085863493 21612 76 -0.12713976972077745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 6.0 18 4.0 19 16.0 20 42.0 21 60.0 22 116.0 23 215.0 24 311.0 25 463.0 26 573.0 27 776.0 28 1040.0 29 1445.0 30 1954.0 31 2651.0 32 3879.0 33 6381.0 34 11795.0 35 22569.0 36 17205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.96923076923077 7.187412587412588 10.011188811188811 59.83216783216784 2 19.302097902097902 6.247552447552448 61.97482517482518 12.475524475524475 3 19.607843137254903 9.485049369248411 61.62904534138905 9.278062152107633 4 18.308578781013118 54.10757741042208 18.900170624597916 8.683673183966881 5 18.456826382478813 16.91141506531286 60.69900142653353 3.9327571256748066 6 21.388512048782534 62.16276695430834 9.90615515866911 6.542565838240025 7 20.709910212301754 15.524041285558447 9.269670778439764 54.49637772370004 8 20.18964504489385 61.968895974937766 5.50194400156639 12.339514978601997 9 19.76168498783251 11.945120416212134 4.188694022544824 64.10450057341053 10-11 20.495230902632095 35.746552543984784 31.272551816732395 12.485664736650723 12-13 21.126681771139268 7.092109311627646 36.30108248720315 35.48012643002993 14-15 21.79029957203994 5.4851612542306505 9.975944728818774 62.74859444491063 16-17 49.51679673295852 30.439008699057368 4.404771894492462 15.639422673491651 18-19 51.13355057971217 11.648088837918351 4.322317170389225 32.89604341198025 20-21 23.68115577186652 5.9851472686078715 4.067718385499706 66.2659785740259 22-23 22.028754440435232 34.224217504405466 4.065620542082739 39.68140751307656 24-25 49.38914802408442 34.246872312006545 4.389602579075085 11.974377084833948 26-27 51.08400704954262 36.46879458416091 6.5460038604638155 5.901194505832658 28-29 22.748666018616294 11.92226192890561 32.07219933842915 33.25687271404894 30-31 20.782018197203982 31.549979369042376 39.80935596444481 7.858646469308828 32-33 45.62988656866722 13.812467655985566 35.6777207435277 4.879925031819517 34-35 25.94737099740561 60.97509807623723 8.215327165544297 4.862203760812862 36-37 19.8714721266538 67.09742384828397 6.671841347095187 6.35926267796705 38-39 20.525999272747615 13.323403541159687 34.395541383457804 31.755055802634892 40-41 20.433566433566433 5.279020979020979 37.41888111888112 36.868531468531465 42-43 48.59196788833645 5.210453073754729 34.99276228837561 11.204816749533219 44-45 22.890968084808815 32.63615003776118 37.419233028446754 7.0536488489832445 46-47 48.173426573426575 7.4356643356643355 12.681818181818183 31.70909090909091 48-49 47.36790579284496 4.9509104640429635 7.538250678302704 40.14293306480938 50-51 24.63567452658667 4.9404212469581275 35.74585326284579 34.67805096360941 52-53 19.33092780621521 5.325725154541132 62.9562809431904 12.387066096053259 54-55 19.511461698765018 5.103567882068281 40.57818990475391 34.806780514412786 56-57 47.46510587116444 4.820844172190988 13.403121591004446 34.31092836564012 58-59 22.365504171416184 7.247005181926894 58.59283761197788 11.79465303467905 60-61 47.08399765041537 33.85080137618528 13.269558893457525 5.79564207994182 62-63 21.465434049873426 67.91443476315017 4.5335030279296795 6.086628159046727 64-65 18.720496807536033 71.05382780975292 4.182022896225689 6.043652486485353 66-67 18.742919481391347 71.05134337543181 4.015440775395461 6.190296367781368 68-69 20.97563704511762 69.25960113003832 4.129255125730748 5.635506699113312 70-71 46.36653520181254 43.59738189141563 4.405471175631451 5.630611731140387 72-73 82.16865616324361 7.755889818952315 4.623743889902868 5.451710127901204 74-75 85.26001454626832 5.069514378426765 4.727537204878594 4.942933870426318 76 85.21497104814121 5.348401353884025 4.523203446250245 4.913424151724524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 3.5 16 8.0 17 11.0 18 13.0 19 14.5 20 16.0 21 18.0 22 24.5 23 32.5 24 34.0 25 34.0 26 59.5 27 110.5 28 116.5 29 97.0 30 107.0 31 133.5 32 177.0 33 204.0 34 251.5 35 305.5 36 368.5 37 425.0 38 451.0 39 545.0 40 747.0 41 1683.5 42 2486.0 43 6014.0 44 21231.0 45 17561.0 46 2202.0 47 1919.0 48 1089.5 49 509.5 50 476.0 51 486.0 52 487.0 53 514.5 54 551.0 55 581.0 56 579.0 57 560.0 58 573.0 59 543.5 60 477.5 61 434.0 62 427.0 63 418.5 64 381.0 65 332.0 66 283.0 67 254.0 68 232.0 69 195.5 70 173.0 71 165.0 72 133.0 73 85.0 74 69.5 75 70.0 76 57.5 77 39.0 78 31.0 79 29.0 80 26.5 81 23.5 82 21.0 83 19.0 84 16.0 85 10.0 86 8.5 87 10.0 88 7.5 89 5.0 90 8.0 91 10.0 92 9.0 93 10.0 94 14.5 95 33.5 96 49.0 97 71.5 98 233.5 99 5284.5 100 10196.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002797124555956477 2 0.002797124555956477 3 0.0 4 0.0 5 0.0 6 0.0013985622779782384 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0013985622779782384 20-21 0.0 22-23 0.0 24-25 0.0048949679729238344 26-27 0.011188498223825907 28-29 0.007692092528880311 30-31 0.010489217084836789 32-33 0.006992811389891192 34-35 0.0020978434169673576 36-37 0.0 38-39 0.0 40-41 0.002797124555956477 42-43 0.0020978434169673576 44-45 0.0 46-47 0.002797124555956477 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.004195686833934715 56-57 0.0 58-59 0.0048949679729238344 60-61 0.0 62-63 0.0013985622779782384 64-65 0.007692092528880311 66-67 0.004195686833934715 68-69 0.0 70-71 0.0 72-73 0.0020978434169673576 74-75 0.00839137366786943 76 0.005594249111912954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 71502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.452770551872675 #Duplication Level Percentage of deduplicated Percentage of total 1 69.8409398066327 9.395541383457804 2 10.292130159060193 2.769153310396912 3 6.34161555255224 2.559368968700176 4 4.699033163530513 2.528600598584655 5 3.1188273209273314 2.0978434169673577 6 2.172783033579374 1.753797096584711 7 1.2579270194406904 1.184582249447568 8 0.6757459195342551 0.727252384548684 9 0.2599022767439443 0.31467651254510365 >10 1.050005198045535 2.5859416519817624 >50 0.08316872855806216 0.8167603703392913 >100 0.13514918390685102 3.8236692679925035 >500 0.02079218213951554 1.6670862353500602 >1k 0.031188273209273316 9.492042180638304 >5k 0.0 0.0 >10k+ 0.02079218213951554 58.2836843724651 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT 31208 43.64633157114486 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 10466 14.637352801320242 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG 3403 4.759307431959945 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC 2354 3.292215602360773 No Hit ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT 1030 1.4405191463175855 RNA PCR Primer, Index 19 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC 685 0.9580151604150933 No Hit CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC 507 0.7090710749349668 No Hit CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT 454 0.6349472742021203 No Hit CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT 330 0.4615255517328187 No Hit ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT 288 0.40278593605773266 No Hit CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC 234 0.3272635730469078 RNA PCR Primer, Index 19 (95% over 21bp) CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG 227 0.31747363710106014 No Hit TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC 216 0.3020894520432995 No Hit TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT 192 0.2685239573718218 RNA PCR Primer, Index 19 (95% over 22bp) TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC 190 0.2657268328158653 No Hit CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT 141 0.19719728119493163 No Hit CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC 127 0.17761740930323627 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG 113 0.15803753741154095 No Hit CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG 111 0.15524041285558446 No Hit CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT 111 0.15524041285558446 No Hit CTCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG 88 0.12307348046208498 No Hit CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT 82 0.11468210679421555 No Hit CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT 81 0.11328354451623732 No Hit TCTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT 72 0.10069648401443317 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.002797124555956477 0.0 5 0.0 0.0 0.0 0.015384185057760623 0.0 6 0.0 0.0 0.0 0.015384185057760623 0.0 7 0.0 0.0 0.0 0.015384185057760623 0.0 8 0.0 0.0 0.0 0.022376996447651815 0.0 9 0.0 0.0 0.0 0.03356549467147772 0.0 10 0.0 0.0 0.0 0.03776118150541244 0.0 11 0.0 0.0 0.0 0.048949679729238346 0.0 12 0.0 0.0 0.0 0.048949679729238346 0.0 13 0.0 0.0 0.0 0.053145366563173056 0.0 14 0.0 0.0 0.0 0.06852955162093367 0.0 15 0.0 0.0 0.0 0.06852955162093367 0.0 16 0.0 0.0 0.0 0.06852955162093367 0.0 17 0.0 0.0 0.0 0.07692092528880311 0.0 18 0.0 0.0 0.0 0.07692092528880311 0.0 19 0.0 0.0 0.0 0.0951022349025202 0.0 20 0.0 0.0 0.0 0.1020950462924114 0.0 21 0.0 0.0 0.0 0.11468210679421555 0.0 22 0.0 0.0 0.0 0.12866772957399794 0.0 23 0.0 0.0 0.0 0.1496461637436715 0.0 24 0.0 0.0 0.0 0.1860087829711057 0.0 25 0.0 0.0 0.0 0.20139296802886633 0.0 26 0.0 0.0 0.0 0.20978434169673577 0.0 27 0.0 0.0 0.0 0.2517412100360829 0.0 28 0.0 0.0 0.0 0.3062851388772342 0.0 29 0.0 0.0 0.0 0.3538362563284943 0.0 30 0.0 0.0 0.0 0.42236580794942796 0.0 31 0.0 0.0 0.0 0.4922939218483399 0.0 32 0.0 0.0 0.0 0.5538306620793824 0.0 33 0.0 0.0 0.0 0.6727084557075327 0.0 34 0.0 0.0 0.0 0.8279488685631171 0.0 35 0.0 0.0 0.0 0.9678050963609409 0.0 36 0.0 0.0 0.0 1.1664009398338508 0.0 37 0.0 0.0 0.0 1.566389751335627 0.0 38 0.0 0.0 0.0 1.9300159436099689 0.0 39 0.0 0.0 0.0 2.3342004419456797 0.0 40 0.0 0.0 0.0 2.6922323851081087 0.0 41 0.0 0.0 0.0 3.079634136108081 0.0 42 0.0 0.0 0.0 3.4488545774943358 0.0 43 0.0 0.0 0.0 3.836256328494308 0.0 44 0.0 0.0 0.0 4.187295460266846 0.0 45 0.0 0.0 0.0 4.557914463931079 0.0 46 0.0 0.0 0.0 4.921540656205421 0.0 47 0.0 0.0 0.0 5.170484741685547 0.0 48 0.0 0.0 0.0 5.492154065620542 0.0 49 0.0 0.0 0.0 5.755083773880451 0.0 50 0.0 0.0 0.0 5.971860926967078 0.0 51 0.0 0.0 0.0 6.194232329165618 0.0 52 0.0 0.0 0.0 6.480937596151157 0.0 53 0.0 0.0 0.0 6.73967161757713 0.0 54 0.0 0.0 0.0 6.990014265335235 0.0 55 0.0 0.0 0.0 7.177421610584319 0.0 56 0.0 0.0 0.0 7.363430393555425 0.0 57 0.0 0.0 0.0 7.51027943274314 0.0 58 0.0 0.0 0.0 7.682302592934463 0.0 59 0.0 0.0 0.0 7.8599200022377 0.0 60 0.0 0.0 0.0 7.999776230035524 0.0 61 0.0 0.0 0.0 8.135436770999412 0.0 62 0.0 0.0 0.0 8.252916002349584 0.0 63 0.0 0.0 0.0 8.43892478532069 0.0 64 0.0 0.0 0.0 8.599759447288188 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGACTA 25 2.8138202E-6 70.00001 24 TCTTAAT 25 2.8138202E-6 70.00001 1 GGCACCC 15 0.0022163575 70.0 7 CTTTACA 35 3.6470738E-9 70.0 1 AAATACA 15 0.0022163575 70.0 2 CCGTCTA 15 0.0022163575 70.0 47 TCTACTG 15 0.0022163575 70.0 50 GTCTACT 15 0.0022163575 70.0 49 TCTTTAT 110 0.0 70.0 1 CTTTTTG 15 0.0022163575 70.0 1 AGGGGGG 3585 0.0 63.751743 70 TTTATAC 135 0.0 62.222225 3 CTTTATA 125 0.0 61.600002 2 TTACACA 40 1.0482836E-8 61.25 3 TTTACAC 40 1.0482836E-8 61.25 2 TCTATAC 35 2.9363764E-7 60.0 1 GACATAT 30 8.300329E-6 58.333332 26 ACATATG 30 8.300329E-6 58.333332 27 ATATGAA 30 8.300329E-6 58.333332 29 AGACTAA 30 8.300329E-6 58.333332 25 >>END_MODULE