FastQCFastQC Report
Wed 25 May 2016
SRR1779910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545250
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280535.1449793672627235No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG71101.3039889958734525No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC12930.23713892709766163Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT7910.14507106831728564No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG7870.14433745988078864No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC200.006950160552.499861
GGCACGC200.006950160552.4998634
AGGACCG200.006950160552.499865
TATGCGC200.006950160552.499863
TCGGTGG11550.045.15139845
GTCGCCG11700.044.87167751
GCCGTAT11450.044.62870854
TGGTCGC11750.044.38286249
TCGCCGT11600.044.35333352
CGGTGGT11850.044.0083246
AGATCTC11700.043.97424340
CTCGGTG11800.043.8981944
GATCTCG11800.043.60157841
ATCTCGG11850.043.41760642
CGTATCA12050.042.98744256
TCTCGGT12300.042.1137143
GGTGGTC12550.042.11144347
GTAGATC12700.041.33847438
CGCCGTA12400.041.2095753
GTATCAT12450.041.0440757