FastQCFastQC Report
Wed 25 May 2016
SRR1779910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545250
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT140232.571847776249427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124442.2822558459422284No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT40720.746813388353966No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG22500.41265474552957354No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC15800.2897753324163228No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT8940.1639614855570839Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC5780.10600641907381936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCA401.0659278E-861.24943546
AGGGGGG29750.055.8818470
CGTCTAC452.7002898E-854.44394348
TATGCCG34050.049.44154443
CCGTCTT32900.049.36124847
GCCGTCT33450.049.2820646
CTCGTAT34350.049.2135239
CGTCTTC33000.049.2116748
TCGTATG34800.049.18058440
CGTATGC34550.049.0299441
CGTATGA506.194023E-848.99955441
TCTCGTA34550.048.9286438
ATCTCGT34600.048.6556237
TGCCGTC34450.048.35949745
ATGCCGT34600.048.35215844
AAGGGGG34250.048.23313569
GTATGCC34850.048.20615842
CTGCTTG33450.048.13109654
TCTTCTG33850.048.07932350
CTTGAAA34650.047.77733657