FastQCFastQC Report
Wed 25 May 2016
SRR1779909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319594
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294069.201048830703956No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG58131.8188701915555363No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC10200.31915492781466487Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG8220.2572013241800535No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT6290.19681220548571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG150.002223568769.99894
ACACGAA200.00694789952.49917613
GGGTCTA407.4680065E-752.4991761
GTTCCCG200.00694789952.4991766
GGGACTA305.873451E-446.6659361
GATCCTA757.530616E-1041.9993441
TCGCCGT11350.040.39584452
TGGTCGC11550.040.30240249
GCCGTAT11300.040.2648654
TCGGTGG11650.040.25687845
GTCGCCG11500.040.17328651
CGGTGGT11650.039.9564546
CTCGGTG11600.039.82696544
CGTATCA11350.039.77911456
TCTCGGT11650.039.35560643
AGATCTC11800.038.85532840
GGTGGTC12200.038.728947
GATCTCG11850.038.69137641
CGCCGTA11950.038.6604853
GTATCAT11850.038.39602357