FastQCFastQC Report
Wed 25 May 2016
SRR1779909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319594
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT146974.598647033423656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104423.267270349255618No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT44671.3977108456354No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG21630.6767961851599217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC16410.5134639573959462No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT9500.2972521386509133RNA PCR Primer, Index 34 (95% over 23bp)
ATCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC4090.12797486811391953No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC3980.12453300124533001No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT3830.11983954642452613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA150.002221400470.0161226
TGTCGGG150.002224160169.9942151
AGGGGGG25350.059.22587270
GTCCGTT200.006941142552.5120932
CGTCTAC200.006949740552.49565548
AAAACGG200.006949740552.49565567
GTCAACA200.006949740552.49565560
CCGTCTT29250.051.56838647
GCCGTCT29550.051.51858546
CGTCTTC29900.051.26666348
CTGCTTG30000.051.21243354
TATGCCG30300.050.93638243
CGTATGC30650.050.81145541
CTCGTAT30650.050.6972739
TCGTATG31550.050.69311540
GTCTTCT29700.050.66921249
TCTTCTG29650.050.6366250
TCTGCTT30300.050.3588753
ATGCCGT30750.050.30478344
CTTCTGC29950.050.2462651