FastQCFastQC Report
Wed 25 May 2016
SRR1779908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351266
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262537.473823256449529No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG55141.5697505594051233No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC9860.2806989574852106Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG7430.21152061400761818No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT6110.1739422545876914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC252.3894638E-455.9965868
GCTAAGT200.00694971552.4967968
TGTACGT200.00694971552.49679612
ATCGCGG200.00694971552.49679641
GGTCCAA352.085241E-549.996951
AGGTACT305.8755657E-446.663825
GGGTCTA305.8755657E-446.663821
GCGATCC305.8755657E-446.663829
CGGCTTA305.8755657E-446.6638225
CTCGGTG9550.043.60990544
TCTCGGT9700.042.93552443
TGGTCGC9800.042.85452749
GCCGTAT9650.042.79531554
TCGGTGG9750.042.7153445
TCGCCGT9900.042.42165452
AGATCTC10000.042.34741640
ATCTCGG9950.042.2084842
CGGTGGT9950.042.2084846
GATCTCG10050.041.78849441
CGCCGTA10050.041.78849453