FastQCFastQC Report
Wed 25 May 2016
SRR1779908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351266
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT116383.313158688856878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97822.7847841806494222No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT48061.368193904334607No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG20610.5867348391247658No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC13110.37322143332972735No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT8360.23799627632620293TruSeq Adapter, Index 10 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC4860.13835668695518494No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC4390.12497651352536254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG24100.057.360670
CGACACT200.006950218752.49583449
GAAGTCG200.006950218752.4958346
AACTCGT200.006950218752.49583437
AAGGGGG27850.049.38567469
ATTATAC8900.049.1604041
CCGTCTT27500.048.359847
CGTCTTC27600.048.31138648
GCCGTCT28050.048.03540846
TATGCCG28350.048.02088543
CTCGTAT28600.047.9682239
ATGCCGT28350.047.7739944
CGTATGC28650.047.76235641
GTATGCC28600.047.72348442
TCGTATG29150.047.66345640
ATCTCGT28550.047.561937
TGCCGTC28300.047.48739645
TCTCGTA28600.047.4787538
CTGCTTG28400.047.4434254
AATCTCG28750.047.35276836
Produced by FastQC (version 0.11.5)