FastQCFastQC Report
Wed 25 May 2016
SRR1779907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194645
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2489812.791492203755556No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG50172.577512908114773No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC9110.4680315446068483Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT5880.3020884173752215No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG5680.2918133011379691No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGT150.002222120570.054
CCCATAG308.354069E-658.3333321
CGCGGTC252.3860874E-456.00000439
AGCGTTC200.00694340652.537
GACCGTA200.00694340652.555
TGTGCGC200.00694340652.53
GTGACAT200.00694340652.55
CGATCTG200.00694340652.543
AAAGCGT200.00694340652.535
TGGCGTA305.8673054E-446.66666424
GTCACAC305.8673054E-446.66666443
GAGCATT305.8673054E-446.66666448
CTTTATA551.3051067E-744.5454522
GCCGTAT8350.043.59281554
TCGCCGT8600.043.13953452
CGCCGTA8650.042.8901753
TCGGTGG8650.042.4855545
GTCGCCG8750.042.451
CGTATCA8700.042.24138356
ATCTCGG8650.042.08092542