FastQCFastQC Report
Wed 25 May 2016
SRR1779907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194645
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT120226.176372370212438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88114.52670245832156No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT44032.262066839631123No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG19671.010557681933777No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC11950.6139381951758329No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT5250.26972180122787637RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3640.1870071155179943No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3580.18392458064681858No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2820.14487913894525933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGC150.00222419269.9835652
ACGTATG150.00222419269.9835640
AGGGGGG18550.061.49498770
TCACATC252.3827971E-456.0156333
ATTATAC5450.053.2902341
GACGCGG200.006949859752.48766717
GCCGTAT200.006949859752.48766747
TGCCGTA200.006949859752.48766746
CCGTCTT21800.051.68510447
CGTATGC22900.051.64725541
CTTGAAA22500.051.63231357
AAGGGGG22100.051.6168369
TGCCGTC22600.051.5586845
CGTCTTC22000.051.53334848
GCCGTCT22050.051.4164946
TATGCCG22750.051.37254343
CTCGTAT23050.051.31115339
TCGTATG23050.051.31115340
ATGCCGT22800.051.25988844
ATCTCGT23100.051.2000937
Produced by FastQC (version 0.11.5)