FastQCFastQC Report
Wed 25 May 2016
SRR1779906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174609
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3368919.293965374064335No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG66293.796482426449954No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC9070.519446305745981Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG7040.4031865482306181No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT6640.38027822162660574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGT150.00222173770.041
TTCGTGG150.00222173770.049
CCGTCGA150.00222173770.044
AGCATGG150.00222173770.025
TCGCCCG200.006942216352.530
AGCGTTC200.006942216352.528
AACCGGA200.006942216352.536
GAGCTTC200.006942216352.51
GTCGAGG200.006942216352.546
CTTCAGA200.006942216352.539
TAAACCG200.006942216352.534
CGACCAC200.006942216352.539
TTTCCGA200.006942216352.551
GCTTGGT200.006942216352.511
TGTGCGC200.006942216352.53
CTTGGTT200.006942216352.512
TGCGCGT200.006942216352.55
CGTCGAG200.006942216352.545
GCCACGG200.006942216352.515
ACACCCG352.0801399E-550.034
Produced by FastQC (version 0.11.5)