FastQCFastQC Report
Wed 25 May 2016
SRR1779906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174609
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113366.492219759577113No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT86804.971106873070689No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT36672.100120841422836No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT15550.8905611967309818No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG9790.5606812936332034No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT6910.39574134208431405No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC5790.3315980275930794No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4110.23538305585622735No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG3250.18613015365760072No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT3150.18040307200659758No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT2480.14203162494487684No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT2360.13515912696367313No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2130.12198683916636599No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT2080.11912329834086444No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT1850.10595101054355734No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC1820.10423288604825638No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT1790.10251476155295546No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT1790.10251476155295546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGCG150.00221975470.01575527
CTACTGG150.002222277769.9957059
CGACATA301.0196345E-769.99570548
CGAATGC301.0196345E-769.99570541
AGCCGTC600.064.1627345
GCCGTAT2050.061.45964446
TAAGACG401.0606527E-861.24624343
AGGGGGG23350.060.70291570
TTAAGCA2150.060.22886752
TCTTAAG1750.059.99632350
GTATTCT352.9625153E-759.99631549
GTCTTCA1700.059.7022249
AAGCCGT1000.059.4963544
ATTATAC9300.059.4587171
CTTAAGC1950.059.2271451
GCCGTCT10900.058.4367846
CGTAATC1500.058.3297548
CCGTCTT11150.058.06818847
GTATTAA1450.057.9274849
TCTCGTA20700.057.31532338