FastQCFastQC Report
Wed 25 May 2016
SRR1779905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280831
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205417.314363442782314No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG40471.4410802226249952No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC7990.284512749660828Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT4570.16273132239674395No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG3940.140297901584939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA150.002224225869.991451
GACTTTC252.3872538E-456.00313663
TTTAGCG200.00694505452.5029426
GGGACGC200.00694505452.5029435
GGACGCG200.00694505452.5029436
TCAAGAA200.00694505452.5029434
ACGCGGG200.00694505452.5029438
CTGGCCA352.0849136E-549.9938964
GCACTTA352.0871117E-549.98499313
TGGTCGC7150.049.93286549
TCGGTGG7100.049.7915245
TCGCCGT7050.049.64816752
CTCGGTG7050.049.64816744
GCCGTAT7100.049.29853454
CGGTGGT7250.049.24413346
GTCGCCG7200.049.09996851
ATCTCGG7200.048.6138342
CGCCGTA7200.048.6138353
TCTCGGT7400.048.2459543
AGATCTC7300.047.9478940
Produced by FastQC (version 0.11.5)