FastQCFastQC Report
Wed 25 May 2016
SRR1779905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280831
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT87873.1289280741798446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71892.5599025748581887No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT41111.463869729481432No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG14880.5298560344121553No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC9080.3233261285256969No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT6590.23466070341237258Illumina PCR Primer Index 11 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC3480.12391794353187505No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT3320.11822056681776584No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2920.10397712503249285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCC150.002223149670.044
GGACCTA150.002223149670.01
GCTCAAG150.002223149670.01
AGGGGGG16450.058.51063570
ATCGTAT252.3879163E-455.99999639
ATATCGT252.3879163E-455.99999637
CGACTTC252.3879163E-455.99999648
CGTCTTC18100.052.98342548
CCGTCTT17750.052.64789247
GCGAGAA200.006946594552.513
GACAACG200.006946594552.570
GCCGACT200.006946594552.546
GTTCCAC200.006946594552.564
CCGACTT200.006946594552.547
GTCTTCA407.463932E-752.549
TATGCCG18350.052.45231643
TCGTATG18800.052.3138340
TCTTCTG18150.052.25895350
GCCGTCT18050.052.16066746
CGTATGC18600.052.12365741