FastQCFastQC Report
Wed 25 May 2016
SRR1779904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111641
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2100818.81745953547532No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG44183.9573275051280445No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC8980.8043639881405577Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG5400.4836932668105804No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT5320.47652744063560876No Hit
TCTTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG1330.11913186015890219No Hit
CTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG1260.11286176225580206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCC150.002219629770.000018
ATATACG150.002219629770.0000135
TCCCGTC150.002219629770.0000112
TATACGT150.002219629770.0000136
CTTAGTC150.002219629770.000016
GTTTCAA252.3816619E-455.99999622
TAGTTCT252.3816619E-455.99999629
TTAATAC200.00693568452.536
GGCGTAA200.00693568452.515
TTCGTAG200.00693568452.54
CCCGTCG200.00693568452.513
CAGGCTC200.00693568452.557
TAGGGAG200.00693568452.55
CATCGTC200.00693568452.562
CGTCGTA200.00693568452.515
ATACGTT200.00693568452.537
CATCCGG200.00693568452.520
CATGACA352.0752654E-550.00000441
GATCCCG305.8564876E-446.66666810
ATCCCGT305.8564876E-446.66666811
Produced by FastQC (version 0.11.5)