FastQCFastQC Report
Wed 25 May 2016
SRR1779904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111641
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT93288.355353320016839No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84567.5742782669449396No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT41843.7477270895101262No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG16051.4376438763536694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC10510.9414104137368888No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT5730.513252299782338TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3420.3063390689800342No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3410.30544334070816276No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3100.277675764280148No Hit
ATTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1970.1764584695586747No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC1640.146899436586917No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT1140.10211302299334474No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT150.002219629770.0000116
CGTTGTT150.002219629770.0000163
GAACGAG150.002219629770.0000164
GCGTTAA207.897341E-570.029
GACGCGT207.897341E-570.026
CGCGTTA207.897341E-570.028
ACGCGTT207.897341E-570.027
ATTATAC6100.064.26231
AGGGGGG18700.061.20320570
GACAACG252.3816619E-455.99999618
AGACGCA252.3816619E-455.99999625
TATCGTA252.3816619E-455.99999638
AAGGGGG21450.052.8671369
GTGTTTC200.00693568452.513
CTATGGA200.00693568452.526
AGACTCT200.00693568452.54
GAGCTTC200.00693568452.544
CGTATAT200.00693568452.518
ACGTTTA200.00693568452.517
GTTTCAA200.00693568452.515
Produced by FastQC (version 0.11.5)